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14. Bacterial Transformation-Regulation with PCR: This activity allows the gene regulation concept to be presented in a more inquiry fashion. Students will transform E. coli with green fluorescent protein gene and will observe the absence of glowing protein. They will hypothesis why the cells did not glow and use PCR (B) to test their hypothesis. After the PCR the students will learn the regulation of this gene, and will induce the promoter to express the product. This activity helps students to visualize regulation and relate this regulation to the lac operon system. Highly recommended for AP Biology and Biotechnology Course. [50 minutes, overnight incubation, and next 50 min class, then PCR (see below) then another one or two 50 min class(es),depending on how much is inquiry, plus a part of a class to complete the activity]

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